Human Islet Research Network

Nanodroplet processing platform for deep and quantitative proteome profiling of 10-100 mammalian cells

Consortia: CBDS

Zhu Y, Piehowski PD, Zhao R, Chen J, Shen Y, Moore RJ, Shukla AK, Petyuk VA, Campbell-Thompson MMathews CE, Smith RD, Qian WJ, Kelly RT.
Nat Commun. 2018 Feb 28;9(1):882.  PMID: 29491378

All An Electrochemical Aptamer-Based Sensor for Real-Time Monitoring of Insulin Analysis of serum Hsp90 as a potential biomarker of β cell autoimmunity in type 1 diabetes Cutting Edge: Early Attrition of Memory T Cells during Inflammation and Costimulation Blockade Is Regulated Concurrently by Proapoptotic Proteins Fas and Bim Single-Cell RNA-Seq of the Pancreatic Islets–a Promise Not yet Fulfilled? Microfluidic device to attain high spatial and temporal control of oxygen Use of human islets to understand islet biology and diabetes: progress, challenges and suggestions Combined Inhibition of DYRK1A, SMAD, and Trithorax Pathways Synergizes to Induce Robust Replication in Adult Human Beta Cells Proinsulin Secretion Is a Persistent Feature of Type 1 Diabetes Human islets expressing HNF1A variant have defective β cell transcriptional regulatory networks New mass spectrometry technologies contributing towards comprehensive and high throughput omics analyses of single cells Comprehensive human cell-type methylation atlas reveals origins of circulating cell-free DNA in health and disease Pancreatic Progenitors: There and Back Again TRPV1 neurons regulate β-cell function in a sex-dependent manner DEXI, a candidate gene for type 1 diabetes, modulates rat and human pancreatic beta cell inflammation via regulation of the type I IFN/STAT signalling pathway Examining How the MAFB Transcription Factor Affects Islet β Cell Function Postnatally Ectonucleoside Triphosphate Diphosphohydrolase-3 Antibody Targets Adult Human Pancreatic? Cells for In Vitro and In Vivo Analysis Endoplasmic reticulum stress alters ryanodine receptor function in the murine pancreatic β cell Oxidative Modifications in Tissue Pathology and Autoimmune Disease Evaluation of different decellularization protocols on the generation of pancreas-derived hydrogels Lack of acute xenogeneic graft- versus-host disease, but retention of T-cell function following engraftment of human peripheral blood mononuclear cells in NSG mice deficient in MHC class I and II expression Targeted demethylation at the CDKN1C/p57 induces human β cell replication Pancreatic islet-autonomous insulin and smoothened-mediated signalling modulate identity changes of glucagon(+) α-cells. PDL1 is expressed in the islets of people with type 1 diabetes and is up-regulated by interferons-α and-γ via IRF1 induction The Role of Accessory Cells in Islet Homeostasis The Effect of Age on the Progression and Severity of Type 1 Diabetes: Potential Effects on Disease Mechanisms Discovering human diabetes-risk gene function with genetics and physiological assays ImmuneDB, a Novel Tool for the Analysis, Storage, and Dissemination of Immune Repertoire Sequencing Data When one becomes many-Alternative splicing in β-cell function and failure 3-D physiomimetic extracellular matrix hydrogels provide a supportive microenvironment for rodent and human islet culture Insulin receptor-mediated signaling regulates pluripotency markers and lineage differentiation Nanowell-mediated two-dimensional liquid chromatography enables deep proteome profiling of <1000 mammalian cells Pancreatic Beta Cell Differentiation From Human Pluripotent Stem Cells Combined Analysis of GAD65, miR-375 and Unmethylated Insulin DNA Following Islet Transplantation in T1D Patients Beta Cell DNA Damage Response Promotes Islet Inflammation in Type 1 Diabetes Development of T cell lines sensitive to antigen stimulation Novel selective thiadiazine DYRK1A inhibitor lead scaffold with human pancreatic β-cell proliferation activity Impaired Store-Operated Calcium Entry and STIM1 Loss Lead to Reduced Insulin Secretion and Increased Endoplasmic Reticulum Stress in the Diabetic β cell Microfluidic-enabled quantitative measurements of insulin release dynamics from single islets of Langerhans in response to 5-palmitic acid hydroxy stearic acid Biomarkers of islet beta cell stress and death in type 1 diabetes Engineering a Single-Agent Cytokine/Antibody Fusion That Selectively Expands Regulatory T Cells for Autoimmune Disease Therapy A Chromatin Basis for Cell Lineage and Disease Risk in the Human Pancreas Quantifying size and diversity of the human T cell alloresponse β Cell dysfunction exists more than 5 years before type 1 diabetes diagnosis Identification and Analysis of Islet Antigen-Specific CD8(+) T Cells with T Cell Libraries Facile carrier-assisted targeted mass spectrometric approach for proteomic analysis of low numbers of mammalian cells. Persistent Elevations in Circulating INS DNA Among Subjects with Longstanding Type 1 Diabetes Serum biomarkers for diagnosis and prediction of type 1 diabetes Development of Kinase-Selective, Harmine-Based DYRK1A Inhibitors that Induce Pancreatic Human β-Cell Proliferation Conventional and Neo-Antigenic Peptides Presented by β Cells Are Targeted by Circulating Naïve CD8+ T Cells in Type 1 Diabetic and Healthy Donors Relief-based feature selection: Introduction and review