Human Islet Research Network

Nanodroplet processing platform for deep and quantitative proteome profiling of 10-100 mammalian cells

Consortia: CBDS

Zhu Y, Piehowski PD, Zhao R, Chen J, Shen Y, Moore RJ, Shukla AK, Petyuk VA, Campbell-Thompson MMathews CE, Smith RD, Qian WJ, Kelly RT.
Nat Commun. 2018 Feb 28;9(1):882.  PMID: 29491378

All Microfluidic-enabled quantitative measurements of insulin release dynamics from single islets of Langerhans in response to 5-palmitic acid hydroxy stearic acid Engineering a Single-Agent Cytokine/Antibody Fusion That Selectively Expands Regulatory T Cells for Autoimmune Disease Therapy β Cell dysfunction exists more than 5 years before type 1 diabetes diagnosis Identification and Analysis of Islet Antigen-Specific CD8(+) T Cells with T Cell Libraries Persistent Elevations in Circulating INS DNA Among Subjects with Longstanding Type 1 Diabetes Development of Kinase-Selective, Harmine-Based DYRK1A Inhibitors that Induce Pancreatic Human β-Cell Proliferation Conventional and Neo-Antigenic Peptides Presented by β Cells Are Targeted by Circulating Naïve CD8+ T Cells in Type 1 Diabetic and Healthy Donors GABA and Artesunate Do Not Induce Pancreatic α-to-β Cell Transdifferentiation In Vivo Thymic tuft cells promote an IL-4-enriched medulla and shape thymocyte development Benchmarking Relief-Based Feature Selection Methods for Bioinformatics Data Mining Learning From Past Failures of Oral Insulin Trial The Dysregulation of the DLK1-MEG3 Locus in Islets From Type 2 Diabetics is Mimicked by Targeted Epimutation of its Promoter with TALE-DNMT Constructs Spatially resolved proteome mapping of laser capture microdissected tissue with automated sample transfer to nanodroplets Hypercholesterolemia induces T cell expansion in humanized immune mice Episodic β-cell death and dedifferentiation during diet-induced obesity and dysglycemia in male mice A Single-Cell Transcriptomic Atlas of Thymus Organogenesis Resolves Cell Types and Developmental Maturation Proteomic Analysis of Single Mammalian Cells Enabled by Microfluidic Nanodroplet Sample Preparation and Ultrasensitive NanoLC-MS Leveraging epigenomics and contactomics data to investigate SNP pairs in GWAS β Cell replacement: improving on the design Postnatal DNA demethylation and its role in tissue maturation Interleukin-6 Reduces β-Cell Oxidative Stress by Linking Autophagy With the Antioxidant Response Advances in drug discovery for human beta cell regeneration High Spatial Resolution Imaging of Mouse Pancreatic Islets Using Nanospray Desorption Electrospray Ionization Mass Spectrometry Methyldopa blocks MHC class II binding to disease-specific antigens in autoimmune diabetes Islet Microvasculature Alterations With Loss of Beta-cells in Patients With Type 1 Diabetes Restructuring of the Gut Microbiome by Intermittent Fasting Prevents Retinopathy and Prolongs Survival in db/db Mice Non-invasive detection of human cardiomyocyte death using methylation patterns of circulating DNA High-Dimensional Phenotyping Identifies Age-Emergent Cells in Human Mammary Epithelia Cystic fibrosis-related diabetes is caused by islet loss and inflammation Improving machine learning reproducibility in genetic association studies with proportional instance cross validation (PICV) Abnormal islet sphingolipid metabolism in type 1 diabetes Modifying Enzymes are Elicited by ER Stress, Generating Epitopes that are Selectively Recognized by CD4+ T Cells in Patients With Type 1 Diabetes Targeted Quantification of Phosphorylation Dynamics in the Context of EGFR-MAPK Pathway Hospital time prior to death and pancreas histopathology: implications for future studies Overexpression of ST5, an activator of Ras, has no effect on β-cell proliferation in adult mice Distinct Gene Expression Pathways in Islets from Individuals with Short- and Long-Duration Type 1 Diabetes Autoimmune Polyendocrine Syndromes Compensation of Signal Spillover in Suspension and Imaging Mass Cytometry Application of a Genetic Risk Score to Racially Diverse Type 1 Diabetes Populations Demonstrates the Need for Diversity in Risk-Modeling Understanding Pre-Type 1 Diabetes: The Key to Prevention Alpha Cell Function and Gene Expression Are Compromised in Type 1 Diabetes Nanodroplet processing platform for deep and quantitative proteome profiling of 10-100 mammalian cells The Role of NOD Mice in Type 1 Diabetes Research: Lessons from the Past and Recommendations for the Future Long-Term Correction of Diabetes in Mice by In Vivo Reprogramming of Pancreatic Ducts Investigating the parameter space of evolutionary algorithms β Cell-Specific Deletion of the IL-1 Receptor Antagonist Impairs β Cell Proliferation and Insulin Secretion. CellCycleTRACER accounts for cell cycle and volume in mass cytometry data α-cell glucokinase suppresses glucose-regulated glucagon secretion Replication confers β cell immaturity Fluctuation Localization Imaging-based Fluorescence in situ Hybridization (fliFISH) for Accurate Detection and Counting of RNA Copies in Single Cells