An integrative single-cell multi-omics profiling of human pancreatic islets identifies T1D associated genes and regulatory signals

Consortia: CBDS, HPAC

Zhao Z, D’Oliveira Albanus R, Taylor H, Tang X, Han Y, Orchard P, Varshney A, Zhang T, Manickam N, Erdos M, Narisu N, Taylor L, Saavedra X, Zhong A, Li B, Zhou T, Naji A, Liu C, Collins F, Parker SC, Chen S.
Res Sq. 2023 Oct 18:rs.3.rs-3343318. doi: 10.21203/rs.3.rs-3343318/v1. PMID:37886586

All Reduction of chemokine CXCL9 expression by omega-3 fatty acids via ADP-ribosylhydrolase ARH3 in MIN6 insulin-producing cells Comparative and integrative single cell analysis reveals new insights into the transcriptional immaturity of stem cell-derived β cells DNA methylation-based assessment of cell composition in human pancreas and islets A fast and sensitive size-exclusion chromatography method for plasma extracellular vesicle proteomic analysis Local dialogues between the endocrine and exocrine cells in the pancreas KD025 is a casein kinase 2 inhibitor that protects against glucolipotoxicity in beta cells SliceChip: a benchtop fluidic platform for organotypic culture and serial assessment of human and rodent pancreatic slices Histone Deacetylase Inhibitors Prevent Cytokine-Induced β Cell Dysfunction Through Restoration of Stromal Interaction Molecule 1 Expression and Activation of Store-Operated Calcium Entry RedRibbon: A new rank-rank hypergeometric overlap for gene and transcript expression signatures Conformational plasticity of RAS Q61 family of neoepitopes results in distinct features for targeted recognition NADPH Oxidase 2-Derived Reactive Oxygen Species Promote CD8+ T Cell Effector Function Protection of β cells against pro-inflammatory cytokine stress by the GDF15-ERBB2 signaling How to Test Human CAR T Cells in Solid Tumors, the Next Frontier of CAR T Cell Therapy Redirecting Human Conventional and Regulatory T Cells Using Chimeric Antigen Receptors Disrupted RNA editing in beta cells mimics early-stage type 1 diabetes RFX6 maintains gene expression and function of adult human islet α cells Simplified homology-assisted CRISPR for gene editing in Drosophila Genetic risk converges on regulatory networks mediating early type 2 diabetes Beta-cell intrinsic dynamics rather than gap junction structure dictates subpopulations in the islet functional network A proteomic meta-analysis refinement of plasma extracellular vesicles Modulation of insulin secretion by RBFOX2-mediated alternative splicing K(ATP) channel activity and slow oscillations in pancreatic beta cells are regulated by mitochondrial ATP production A versatile pumpless multi-channel fluidics system for maintenance and real-time functional assessment of tissue and cells Checkpoint kinase 2 controls insulin secretion and glucose homeostasis HNF1α maintains pancreatic α and β cell functions in primary human islets Stress-induced β cell early senescence confers protection against type 1 diabetes Human Pseudoislet System for Synchronous Assessment of Fluorescent Biosensor Dynamics and Hormone Secretory Profiles Inhibition of polyamine biosynthesis preserves β cell function in type 1 diabetes Dynamic scRNA-seq of live human pancreatic slices reveals functional endocrine cell neogenesis through an intermediate ducto-acinar stage An integrative single-cell multi-omics profiling of human pancreatic islets identifies T1D associated genes and regulatory signals A bright future for glucagon and alpha cell biology Exocrine pancreas in type 1 and type 2 diabetes: different patterns of fibrosis, metaplasia, angiopathy, and adiposity Benchmarking algorithms for joint integration of unpaired and paired single-cell RNA-seq and ATAC-seq data Functional interrogation of twenty type 2 diabetes-associated genes using isogenic hESC-derived β-like cells HLA3DB: comprehensive annotation of peptide/HLA complexes enables blind structure prediction of T cell epitopes Deconstructing the integrated oscillator model for pancreatic β-cells The leptin receptor has no role in delta-cell control of beta-cell function in the mouse Transcriptome and secretome profiling of sensory neurons reveals sex differences in pathways relevant to insulin sensing and insulin secretion Insulin-like Growth Factor-1 Synergizes with IL-2 to Induce Homeostatic Proliferation of Regulatory T Cells PAX4 loss of function increases diabetes risk by altering human pancreatic endocrine cell development Generation and molecular characterization of human pluripotent stem cell-derived pharyngeal foregut endoderm Multiplexed CRISPR gene editing in primary human islet cells with Cas9 ribonucleoprotein Interferon-α promotes neo-antigen formation and preferential HLA-B-restricted antigen presentation in pancreatic β-cells Scalable generation of 3D pancreatic islet organoids from human pluripotent stem cells in suspension bioreactors Systematic review of type 1 diabetes biomarkers reveals regulation in circulating proteins related to complement, lipid metabolism, and immune response Fluorescein-based sensors to purify human α-cells for functional and transcriptomic analyses The beta cell-immune cell interface in type 1 diabetes (T1D) Complementary Approaches to Interrogate Mitophagy Flux in Pancreatic β-Cells Fatty acid-mediated signaling as a target for developing type 1 diabetes therapies Beta cell primary cilia mediate somatostatin responsiveness via SSTR3
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