CHIB Shared Resources

Glucose Stimulated Insulin Release (GSIR)

NIH Clinical Islet Transplantation Consortium SOP for GSIR, originally CellR4:

Functional Assessment of Purified Human Pancreatic Islets: Glucose Stimulated Insulin Release by ELISA – A Standard Operating Procedure of the NIH Clinical Islet Transplantation Consortium. CellR4 2014; 2 (2): e900, http://www.cellr4.org/article/900
NIH_CIT_SOP1-Islet-GSIR.pdf (2 downloads )

SOPs for Islet Processing and Evaluation:

Purified Human Pancreatic Islet: Qualitative and Quantitative Assessment of Islets Using Dithizone (DTZ) – Standard Operating Procedure of the NIH Clinical Islet Transplantation Consortium (SOP 3104 A01, July 7, 2008). CellR4 2015; 3 (1): e1369, http://www.cellr4.org/article/1369
In Vivo Assessment of Human Islet Potency. CellR4 2014; 2 (4): e1150, http://www.cellr4.org/article/1150

Standard GSIR protocol recommended by the Integrated Islet Distribution Program (IIDP)

The Standard Operating Procedure for Potency Test: Glucose Stimulated Insulin Release Assay, SOP #: QA-005-01. https://iidp.coh.org/docs/SOPs/QA/QA_005_01.pdf IIDP_SOP_GSIR-QA_005_01.pdf (0 downloads )

GSIR protocols use a 2.8 mM low and a 28 mM high glucose solution (1 h, 37°C) for testing followed by insulin ELISA (Mercodia) for evaluation.

HIRN Assay Summary

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