Human Islet Research Network

Project Abstracts - HPAC

Penn Integrated Human Pancreas Analysis Program

Contact PI: Ali Naji, MD, PhD, University of Pennsylvania (UC4 DK112217)

Klaus Kaestner, PhD, Investigator, University of Pennsylvania
Michael Feldman, MD, PhD, co-Investigator, University of Pennsylvania
Jason Moore, PhD, co-Investigator, University of Pennsylvania
Michael Betts, PhD, co-Investigator, University of Pennsylvania
Doris Stoffers, MD, PhD, co-Investigator, University of Pennsylvania


Summary of Project Abstract & Publications

Start Date: September 20, 2016



The UC4DK112217 team headed by Dr. Ali Naji at the University of Pennsylvania combines expertise ranging from pancreas procurement and islet isolation to data integration and analysis, supporting the Human Pancreas Analysis Program (HPAP) through six cores. Core A (headed by Dr. Naji) will procure a spectrum of human pancreata and detailed donor medical history in close collaboration with Dr. Atkinson’s team from the University of Florida; perform high resolution HLA typing by next generation sequencing; isolate islets; and distribute islets and tissues to the other Cores for further analysis or processing. Core B (headed by Dr. Stoffers) will perform physiological phenotyping on the isolated islets, which will also be coordinated closely with Dr. Powers’ group at Vanderbilt University. Core C (headed by Dr. Betts) will quantify and characterize memory T cell subsets by flow cytometry and single cell analysis; characterize suppressive activity of Tregs and the ability of related effector cells to be suppressed; perform B cell phenotyping; and generate chromatin accessibility maps of enhancers in pathogenic immune cell types. Core D (Dr. Kaestner) will perform multiple advanced modalities for the molecular profiling of isolated islets including RNAseq and microRNAseq of sorted islet cell populations; mass cytometry for single cell quantification of more than 20 cell surface and intracellular markers; and single cell RNAseq. Core E (Dr. Feldman) will process tissues and analyze them using advanced technologies such as multiplexed immunoflourescent staining, whole slide imaging, quantitative image analysis of protein markers and immune cell infiltrates, and collaborate with Dr. Powers’ team to integrate their advance imaging modalities. This Core will also develop 2-dimensional mass cytometry to pancreatic sections using laser ablation technology. Finally, Core F (headed by Dr. Moore) will assemble, annotate and maintain an open access database for the Program and its member-researchers, and collaborate with the HIRN in the sharing of data from both UC4 programs.

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